M. J. Ziegler, K. Yserentant, V. Middel, V. Dunsing, A. J. Gralak, K. Pakari, J. Bargstedt, C. Kern, S. Chiantia, U. Strähle, D.-P. Herten* and R. Wombacher*
“A chemical strategy to control protein networks in vivo”

bioRxiv, 2020, doi: 10.1101/2020.04.08.031427, (Link)

M. J. Ziegler and R. Wombacher*
“Light-induced protein proximity by activation of gibberellic acid derivatives in living cells”
Methods in Enzymology, 2020, (Link)

M. Baalmann, L. Neises, S. Bitsch, H. Schneider, L. Deweid, N. Ilkenhans, M. Wolfring, M. J. Ziegler, P. Werther, J. Wilhelm, H. Kolmar and R. Wombacher*
“A Bioorthogonal Click Chemistry Toolbox for Targeted Synthesis of Branched and Well-Defined Protein-Protein Conjugates”

ChemRxiv, 2019, doi: 10.26434/chemrxiv.10743344.v1, (Link)

M. Baalmann, L. Neises, S. Bitsch, H. Schneider, L. Deweid, P. Werther, N. Ilkenhans, M. Wolfring, M. J. Ziegler,  J. Wilhelm, H. Kolmar and R. Wombacher*
“A Bioorthogonal Click Chemistry Toolbox for Targeted Synthesis of Branched and Well-Defined Protein-Protein Conjugates”

Angew. Chem Int. Ed., 2020, 59, 12885 –12893.

D. Ollech, T. Pflästerer, A. Shellard, C. Zambarda, J. P. Spatz, P. Marcq, R. Mayor, R. Wombacher* and E. A. Cavalcanti-Adam*
“An optochemical tool for light-induced dissociation of adherens junctions to control mechanical coupling between cells”
Nat. Commun., 2020, 11, 472, (Link)

P. Werther, K. Yserentant, F. Braun, N. Kaltwasser, C. Popp, M. Baalmann, D.-P. Herten* and R. Wombacher*
“Live-Cell Localization Microscopy with a Fluorogenic and Self-Blinking Tetrazine Probe”

Angew. Chem. Int. Ed., 2020, 59, 804, (Link)


J.S. Möhler, P. Werther and R. Wombacher*
“Proximity-Induced Bioorthogonal Chemistry Using Inverse Electron Demand Diels-Alder Reaction”
In Proximity Labeling: Methods and Protocols, M. Sunbul, and A. Jäschke, eds. (New York, NY: Springer New York), 2019, pp. 147-163, (Link)

M. Baalmann, M.J. Ziegler, P. Werther, J. Wilhelm and R. Wombacher*
“Enzymatic and Site-Specific Ligation of Minimal-Size Tetrazines and Triazines to Proteins for Bioconjugation and Live-Cell Imaging”
Bioconjugate Chemistry, 2019, 30, 1405-1414. (Link)


M. Baalmann, M. Best, R. Wombacher*
“Site-Specific Protein Labeling Utilizing Lipoic Acid Ligase (LplA) and Bioorthogonal Inverse Electron Demand Diels-Alder Reaction”
In Noncanonical Amino Acids: Methods and Protocols, E.A. Lemke, ed. (New York, NY: Springer New York), 2018, pp. 365-387, (Link)

P.Werther, J. S. Möhler and R. Wombacher*
“A Bifunctional Fluorogenic Rhodamine Probe for Proximity-Induced Bioorthogonal Chemistry”
Chem. Eur. J., 2017, 72, 18216–18224. (Link)


K. M. Schelkle, C. Schmid, K. Yserentant, M. Bender, I. Wacker, M. Petzoldt, M. Hamburger, D.-P. Herten, R. Wombacher, R. R. Schröder, U. H. F. Bunz
“Cell Fixation by Light-Triggered Release of Glutaraldehyde”
Angew. Chem. Int. Ed., 2017, 56, 4724-4728.(Link)
Angew. Chem., 2017, 129, 4802-4806. (Link)


A. Wieczorek, P. Werther, J. Euchner and R. Wombacher*
“Green- to Far-red-emitting Fluorogenic Tetrazine Probes – Synthetic Access and No-wash Protein Imaging Inside Living Cells “
Chem. Sci., 2017,8, 1506-1510. (Link)


S. Hauke, A. von Appen, T. Quidwai, J. Ries and R. Wombacher*
“Specific Protein Labeling with Caged Fluorophores for Dual-Color Imaging and Super-Resolution Microscopy in Living Cells”
Chem. Sci., 2017, 8 (1), 559-566. (Link)


M. Best, I. Porth, S. Hauke, F. Braun, D.-P. Herten and R. Wombacher*
“Protein-Specific Localization of a Rhodamine-Based Calcium-Sensor in Living Cells”
Org. Biomol. Chem., 2016, 14, 5606-5611. (Link)


M. Baalmann und R. Wombacher*
Trendbericht Biochemie: “Dimerisierung von Proteinen chemisch induzieren”
Nachrichten aus der Chemie, 2016, 64, 3, 305-309.


M. Best, A. Degen, T.T. Schmidt, M. Baalmann and R. Wombacher*
“Two-Step Protein Labeling by Using Lipoic Acid Ligase with Norbornene Substrates and Subsequent Inverse-Electron Demand Diels–Alder Reaction”.
ChemBioChem., 2015, 16, 1158-1162


K. M. Schelkle, T. Griesbaum, D. Ollech, S. Becht, T. Buckup, M. Hamburger und R. Wombacher*
“Lichtinduzierte Proteindimerisierung in lebenden Zellen durch Ein- und Zweiphotonenaktivierung von Gibberellinsäurederivaten”.
Angew. Chem., 2015, 54, 2867-2871

K. M. Schelkle, T. Griesbaum, D. Ollech, S. Becht, T. Buckup, M. Hamburger and R. Wombacher*
“Light-Induced Protein Dimerization by One- and Two-Photon Activation of Gibberellic Acid Derivatives in Living Cells”.
Angew. Chem. Int. Ed., 2015, 54, 2825-2829


Cover Picture (Angew. Chem. Int. Ed. 9/2015)
Precise spatiotemporal control of intracellular protein dimerization by using light as an external stimulus is possible with the phytohormone gibberellic acid. As R. Wombacher et al. show in their Communication on page 2825 ff., irradiation of gibberellic acid (closed lock) with light or photons sets free the biological activity (open lock) through a one- or two-photon absorption process. The induced conformation change and dimerization is symbolized by the embracing couple (a wood cut by the artist Edvard Munch, 1863–1944).

Click here to see a movie of spatiotemporal control of protein dimerisation within a living cell (localisation of GFP to mitochondria in COS7 cells) using caged GA3 activated by a light pulse.

S. Hauke, M. Best, T. T. Schmidt, M. Baalmann, A. Krause and R. Wombacher*
“Two-Step Protein Labeling Utilizing Lipoic Acid Ligase and Sonogashira Cross-Coupling”.
Bioconjugate Chem., 2014, 25 (9), 1632–1637.


A.Wieczorek, T. Buckup and R. Wombacher*
“Rigid tetrazine fluorophore conjugates with fluorogenic properties in the inverse electron demand Diels–Alder reaction”.
Org. Biomol. Chem. 2014, 12, 4177-4185.


E. Gatzogiannis ,  Z. Chen ,  L. Wei ,  R. Wombacher ,  Y. Kao ,  G. Yefremov ,  V. W. Cornish and W. Min
“Mapping protein-specific micro-environments in live cells by fluorescence lifetime imaging of a hybrid genetic-chemical molecular rotor tag”.
ChemComm 2012, 48, 8694-8696


R. Wombacher* and V. W. Cornish
“Chemical tags: Applications in live cell fluorescence imaging”.
J Biophotonics 2011, 4,  391-402. Editor’s choice: free access

A. A. Hoskins, L. J. Friedman, S. S. Gallagher, D. J. Crawford, E. G. Anderson, R. Wombacher, N. Ramirez, V. W. Cornish, J. Gelles and M. J. Moore
“Ordered and dynamic assembly of single spliceosomes”.
Science 2011, 331, 1289-95.
Article highlighted in:  C&EN, March 21, 2011; Nat Struct Mol Biol, April issue, 2011.


R. Wombacher, M. Heidbreder, S. van de Linde, M. P. Sheetz, M. Heilemann, V. W. Cornish and M. Sauer
Live-cell super-resolution imaging with trimethoprim conjugates”.
Nat Methods 2010, 7, 717-9.


S. S. Gallagher, C. Jing, D. S. Peterka, M. Konate, R. Wombacher, L. J. Kaufman, R. Yuste and V. W. Cornish
“A trimethoprim-based chemical tag for live cell two-photon imaging”.
Chembiochem 2010, 11, 782-4.

R. Wombacher and A. Jaeschke:
“Probing the active site of a diels-alderase ribozyme by photoaffinity cross-linking”.
J Am Chem Soc 2008, 130, 8594-5. Article evaluated in Faculty1000.

A. M. Chiorcea-Paquim, J. A. Piedade, R. Wombacher, A. Jaeschke and A. M. Oliveira-Brett
Atomic force microscopy and anodic voltammetry characterization of a 49-mer diels-alderase ribozyme”.
Anal Chem 2006, 78, 8256-64.

R. Wombacher, S. Keiper, S. Suhm, A. Serganov, D. J. Patel and A. Jaeschke
“Control of stereoselectivity in an enzymatic reaction by backdoor access”.
Angew Chem Int Ed Engl 2006, 45, 2469-72.

R. Wombacher,  A. Jaeschke:
“Catalytic ribonucleic acids”.
BIOspektrum 2006, 12,  619-621.

A. Serganov, S. Keiper, L. Malinina, V. Tereshko, E. Skripkin, C. Hobartner, A. Polonskaia, A. T. Phan, R. Wombacher, R. Micura, Z. Dauter, A. Jaeschke and D. J. Patel
“Structural basis for Diels-Alder ribozyme-catalyzed carbon-carbon bond formation”.
Nat Struct Mol Biol 2005, 12, 218-24. Article evaluated in Faculty1000.

W. C. Kurschat, J. Muller, R. Wombacher and M. Helm
“Optimizing splinted ligation of highly structured small RNAs”.
RNA 2005, 11, 1909-14.

A. Jaeschke, F. Stuhlmann, D. Bebenroth, S. Keiper and R. Wombacher
“Ribozyme-catalysed carbon-carbon bond formation”.
Biochem Soc Trans 2002, 30, 1137-40.